Curing of a plasmid fromE.coliusing high-voltage electroporation
نویسندگان
چکیده
منابع مشابه
High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA.
Electroporation permits the uptake of DNA by mammalian cells and plant protoplasts because it induces transient permeability of the cell membrane. We investigated the utility of high-voltage electroporation as a method for genetic transformation of intact bacterial cells by using the enteric pathogen Campylobacter jejuni as a model system. This report demonstrates that the application of high-v...
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ABSTRACT The curing of five medicinal plant extracts (Satureia hortensis, Glycyrrhiza glabra, Teucrium Pollium, Myrtus Communis, Eukalypetus globolus) on plasmid bearing strains of klebsilla pnenmoniae resistance to multiple antibiotics were investigated. Alcoholic and maceration extraction were carried out from leaves, fruits or roots of the above plants. Mimimum inhibitory concentrations (MI...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.23.10131